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IOL 2120 – Student Lab Manual 6th ed. Inoculate the following with your unknown: 1 nutrient agar slant (serves as a backup) 1 nutrient broth (serves as a backup) 1 phenol-red glucose broth 1 phenol-red lactose broth 1 phenol-red mannitol broth 1 nitrate broth 6 items total: Incubate all at 37 ºC Store your original unknown slant culture at 5ºC. • Phenol Red. • HEPES. The complete formulation is available. Using DMEM DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and...